Fig. 5.

Sperm DNA oxidation in aging Prdx6^−/− and WT mice. (A) Immunocytochemistry showing 8-OHdG labeling in mouse spermatozoa. Cauda epididymis spermatozoa were permeabilized with methanol and incubated overnight with the anti-8-OHdG antibody and then with the biotin-labeled anti-mouse antibody followed by streptavidin conjugated to Alexa Fluor 555. All pictures were taken at 1000× magnification using the same exposure time. No labeling was observed with secondary antibody alone (data not shown). (B) Percentage of spermatozoa showing DNA oxidation (8-OHdG labeling). ^# Means higher than the other age-matched group and ^## means higher than the other age groups of WT males (p<0.001). N=10 for each group and genotype.