Fig. 2.

Concentration and viability time-course measurement of TB, PI, and AO/PI-stained Jurkat cells. a Viabilities of each method were measured from 0 to 168 h experimental duration. At the 24 h time point, the viability measured by TB showed approximately 80 %, while the AO/PI or PI showed 70 %. b The total concentrations were also measured by AO/PI and PI methods, which showed consistent results, whereas the TB method showed a decrease over time. c The live cell concentrations were measured and showed no differences between the two methods, however d the dead cells showed higher concentrations for AO/PI and PI methods than the TB method over time