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. Author manuscript; available in PMC: 2015 Mar 24.
Published in final edited form as: Biochem Pharmacol. 2004 Nov 15;68(10):2075–2086. doi: 10.1016/j.bcp.2004.07.014

Fig. 3.

Fig. 3

Concentration-response curves of P2Y1-transfected COS-7 cells and P2Y1/6 chimeric receptors with exchanges in multiple receptor domains. P2Y1 receptors (squares) or chimeric receptors 284–298 (triangles), 110–133/284–298 (circles), or 37–61/110–133/284–298 (diamonds) were transiently expressed in COS-7 cells. [3H]-Inositol phosphate accumulation was measured following a 30-min incubation with increasing concentrations of treated UDP in the presence of 10 mM LiCl (see Section 2 for details). Maximal responses in fold increase of [3H]-inositol phosphate accumulation compared to basal were 2-fold for P2Y1 receptors, 2.5-fold for construct 284–298, 2.8-fold for construct 110–133/284–298, and 3.8-fold for construct 37–61/110–133/284–298. The basal counts for the P2Y6 receptor were 320 cpm and stimulated counts were 1300 cpm. Concentration response curves show a single representative experiment of three independent experiments.