Table 3.
Influence of environmental stressors on ethanol preference during the limited-access 2-bottle choice paradigm.
| Restraint | Tail Suspension |
Predator Odor 1 |
Predator Odor 2 |
Foot Shock | Tail Pinch | |
|---|---|---|---|---|---|---|
| Females | ||||||
| Baseline | .87±.04 (9) | .95±.03 (8) | .90±.03 (10) | .83±.07 (12) | .92±.03(12) | .94±.02(12) |
| Stress | .63±.13(9)+ | .85±.10(8) | .73±.13(10) | .45±.07 (12)*** | .82±.06 (12) | .79±.07(12)+ |
| Post Day 1 | .87±.07 (9) | .94±.06 (8) | .95±.04(10) | .85±.06 (12) | .85±.05 (12)+ | .96±.10(12) |
| Post Day 2 | .80±.08 (9) | .92±.06 (8) | .98±.02(10) | .91±.04 (12) | .94±.05 (12) | .85±.04(12)* |
|
| ||||||
| Males | ||||||
| Baseline | .87±.08 (6) | .96±.04 (6) | .91±.04 (9) | .92±.03 (12) | .93±.03 (12) | .98±.02(10) |
| Stress | .43±.14(6)* | .86±.09 (6) | .57±.12(9)* | .72±.06 (12)* | .90±.08 (12) | .92±.06(10) |
| Post Day 1 | .64±.13(6) | 1.0±.01 (6) | .96±.04 (9) | .90±.06 (12) | .89±.05 (12) | .97±.03 (10) |
| Post Day 2 | .89±.ll (6) | .92±.08 (6) | .93±.05 (9) | .91±.04 (12) | .96±.04 (12) | .89±.05 (10) |
Mean (±SEM) of ethanol preference in female and male mice following 30-min restraint (Cohort 1), 6-min tail suspension (Cohort 1), 30-min predator odor (1= Cohort 1, 2= Cohort 2), 5-min intermittent foot shock (Cohort 2), and 15-min tail pinch stress (Cohort 2). Baseline is the average of the two drinking days immediately prior to stress day. Group size is given in parentheses; only those animals that consumed fluid during the limited access procedure across all days of interest were included (as no fluid consumed indicates that preference could not be calculated).
p<0.001
p<0.01
p<0.05
p<0.09 vs. respective baseline preference (post hoc t-test).