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. Author manuscript; available in PMC: 2015 Mar 24.
Published in final edited form as: ACS Chem Biol. 2013 Oct 29;9(1):247–257. doi: 10.1021/cb400740c

Figure 2.

Figure 2

Discovery of girolline as a potential inhibitor for TLR5 signalling from marine sponge extracts. (a) The first round of screening was performed on the crude extracts to identify potentially active extract fractions. (b) The second round was confirmatory examining dose-responsiveness and non-flagellin stimulated samples were used to investigate false positive results due to compound toxicity. X denotes extracts excluded from further analysis due to potential toxicity. (c–d) The confirmed positive crude extracts underwent further assay-guided fractionation until a pure compound was identified (e). Flg: flagellin.