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. 2015 Mar 24;6:179. doi: 10.3389/fpls.2015.00179

Figure 8.

Figure 8

Interaction of GmDi19-5 with GmLEA3.1. (A) Yeast two-hybrid interaction assay. Vectors were co-introduced into yeast in different combinations: 1, pGADT7 and pGBKT7; 2, pGBKT7-GmDi19-5 and pGADT7; 3, pGBKT7 and pGADT7-GmLEA3.1; 4, pGBKT7-GmDi19-5 and pGADT7-GmLEA3.1. Transformants were placed on selection medium and grown for 4 d. (B) In vivo BiFC assay. The expression of GmDi19-5 alone (YFPN-GmDi19-5 + pSPYCE) was used as the control.