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. 2015 Mar 24;6:179. doi: 10.3389/fpls.2015.00179

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Copyright © 2015 Feng, Cui, Cui, Chen, Yang, Ma, He and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction of GmDi19-5 with GmLEA3.1. (A) Yeast two-hybrid interaction assay. Vectors were co-introduced into yeast in different combinations: 1, pGADT7 and pGBKT7; 2, pGBKT7-GmDi19-5 and pGADT7; 3, pGBKT7 and pGADT7-GmLEA3.1; 4, pGBKT7-GmDi19-5 and pGADT7-GmLEA3.1. Transformants were placed on selection medium and grown for 4 d. (B) In vivo BiFC assay. The expression of GmDi19-5 alone (YFP^N-GmDi19-5 + pSPYCE) was used as the control.