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. 2015 Mar 24;6:190. doi: 10.3389/fmicb.2015.00190

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Copyright © 2015 Pohlschroder and Esquivel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A subset of H. volcanii adhesion pilins promotes microcolony formation while a distinct set appears to inhibit this process. Liquid cultures of H. volcanii ΔpilA[1-6] or wild-type with an expression vector (control) or the plasmid expressing individual His-tagged PilA pilins, were incubated overnight in liquid media with plastic cover slips to assay surface adhesion using the modified Ali assay (O’Toole and Kolter, 1998; Tripepi et al., 2010). Light micrographs of the coverslips taken at 35× magnifications are shown. Modified image from Esquivel et al. (2013).