Skip to main content
. 2015 Mar 24;6:220. doi: 10.3389/fmicb.2015.00220

FIGURE 4.

FIGURE 4

Puumala virus infection of HUVEC induces tissue factor expression resulting in enhanced thrombin generation. MOI 3 infection resulted in increased TF expression (A, Mann–Whitney U p = 0.0002) on HUVEC surface after 48 h (A) and in the cell lysate (B). Thrombin generation time (TGT) was significantly decreased, indicating more thrombin formation, for cells infected with PUUV (MOI 3) at 24 (C) and 48 (D) hours post infection (one way ANOVA; 24 h p < 0.01 and 48 h p < 0.001). Results are shown with whiskers from minimum to maximum. MOI 0.5 infection resulted in shortened TGT 48 h post infection (one way ANOVA p < 0.001). Mean tissue factor (TF) concentration, calculated from TGT standard curve, increased more than ninefold after MOI 3 infection (one way ANOVA 24 and 48 h p < 0.01). MOI 0.5 infection increased TF concentration threefold after 48 h (one way ANOVA p < 0.05; E, F; bars represent SE of the mean). Data are representative of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p ≤ 0.001.