Table 1.

Characteristics of PCR primers, nucleotide substitutions, restriction enzymes, amino acid changes, PCR producte size, and digested sequence sizes (allele) sizes for RFLP polymorphisms in Exon 2 of the Ovar-DRB1 gene.

Characteristic	Palindrome 2	Palindrome 2
Primer sequence	5-TAT CCC GTC TCT GCA GCA CAT TTC-3
	5-TCG CCG CTG CAC ACT GAA ACT CTC-3
Nucleotide substitution	(CTGCAG to GTGCAG or TTGCAG)	(TCGA to TACG)
Restriction enzyme	PstI	TaqI
Amino acid change	Phe and Tyr to Val and Cys	Phe to Tyr
PCR product(bp)	285	285
Allele size(bp)	(+/+): 241 and 44	(+/+): 163 and 122