Figure 1.
Maximum-likelihood phylogeny of V. parahaemolyticus clinical and environmental isolates analyzed in this study compared to V. parahaemolyticus isolates that have been previously characterized by complete or draft genome sequencing and are available in the public domain. The nucleotide sequences of seven conserved genes (recA, gyrB, pyrC, dtdS, tnaA, dnaE, and pntA) were concatenated for each V. parahaemolyticus isolate, and V. campbellii ATCC BAA-1116 was included as an outgroup. The phylogeny was constructed using RAxML (Stamatakis, 2006) with 100 bootstrap replications, and visualized using FigTree v1.3.1 (http://tree.bio.ed.ac.uk/software/figtree/). Only bootstrap values ≥50 are shown. The scale bar represents 0.03 nucleotide substitutions per site. The genomes that were sequenced in this study or in previous studies that are available in the public domain are indicated in bold and are listed in Table 2. The post-1995 V. parahaemolyticus O3:K6 isolates are indicated by an orange box, the O4:K12 isolates are indicated by a purple box, and the O4:K8 isolates are indicated in yellow. The V. parahaemolyticus isolates obtained from environmental sources are indicated in green, while the isolates from clinical sources are indicated in black. The presence of the virulence-associated thermostable direct hemolysins, tdh and trh, in each of the genomes is indicated by symbols.