Skip to main content
. Author manuscript; available in PMC: 2015 Mar 24.
Published in final edited form as: Methods Mol Biol. 2011;699:119–164. doi: 10.1007/978-1-61737-950-5_7

Fig. 9.

Fig. 9

Inhibition of Teff proliferation by Treg cells. Teff cells were stained with CFSE and incubated with graded ratios of Treg cells in the presence of anti-CD3, anti-CD28, and accessory cells, as described in Fig. 8. The maximum proliferative potential of Teff was assessed in the absence of Treg cells (Treg:Teff ratio of 0:1). As increasing numbers of Tregs were added to the culture system, increasing inhibition of Teff cell proliferation was observed, as expected. Similar results were obtained with both CellVue Claret-stained (solid line) or unstained (dashed line) Treg, indicating that staining with the CellVue Claret tracking dye did not affect the potency of inhibition by Treg cells. Proliferative fraction (%P) and Proliferative Index (PI) were determined as described in Fig. 8 and Subheading 3.4.5. Data points in (b) and (c) represent the mean ± 1 standard deviation of triplicate samples. (a) Representative proliferation profiles for Teff at varying Treg:Teff ratios (filled histograms), showing increasing inhibition of proliferation at higher ratios. Unstained, unstimulated cells (unfilled histograms) are overlaid for reference. Stained, unstimulated cells largely overlapped with the stimulated parental population (not shown in this figure; see Fig. 1c). (b) Effect of Treg:Teff ratio on Proliferative Fraction of Teff. (c) Effect of Treg:Teff ratio on Proliferative Index of Teff.