. Author manuscript; available in PMC: 2015 Mar 24.

Published in final edited form as: Methods Mol Biol. 2011;699:119–164. doi: 10.1007/978-1-61737-950-5_7

Table 1.

Non-Perturbing Membrane-Dye Staining Conditions for Selected Cell Types

Cell type	Final cell concentration	Final dye concentration	Reference(s)
hPBMC^a(high cell #)	1 × 10⁷cells/mL	2 µM PKH67	(49)
	3 × 10⁷cells/mL	4 µM CellVue Claret	(45)
	5 × 10⁷cells/mL	5 µM CellVue Claret	Fig. 6

hPBMC^a(low cell #)	5 × 10⁶cells/mL	2 µM PKH26	(50)
hPBMC^a(low cell #)	1 × 10⁶cells/mL	1 µM CellVue Claret	Figs. 8 and 10

Cultured cell lines	1 × 10⁷/mL	15 µM PKH26 (U937)	Fig. 4b
	1 × 10⁷/mL	12.5–15 µM PKH26 (U937)	(41)
	1 × 10⁷/mL	1 µM PKH67 (K562)	Fig. 5
	1 × 10⁷/mL	1 µM PKH67 (polyclonal T cell lines)	(15)
	1 × 10⁷/mL	10 µM CellVue Claret (YAC-1)	E. Breslin, personal communication

A low-speed wash (300 × g) post-Ficoll–Hypaque was used to minimize platelet contamination (see Note 11)