Figure 6.

Distribution of Ploidy Levels during Leaf Development and Nuclei Area of BSC and MC between G. gynandra and T. hassleriana.

(A) Ploidy distribution of developing leaf (category 0 till 5) in percentage in G. gynandra and T. hassleriana. Measurements performed in n = 3 (except G0 = 1 replicate). For each replicate, at least 2000 nuclei were measured by flow cytometry.

(B) Quantification of BSC and MC nuclei area in cross sections (n = 3 ± se) of mature G. gynandra and T. hassleriana leaves (stage 5). Area of nuclei in µm² with at least 150 nuclei analyzed per cell type per species per replicate. Asterisks indicate statistically significant differences between BSC and MC (***P value < 0.001); n.s., not significant.

(C) Fluorescence microscopy images of propidium iodide-stained leaf cross sections (stage 5) of T. hassleriana (left) and G. gynandra (right). Arrowheads point to nuclei of the indicated cell type. V, vein; S, stomata. Bar = 50 μm.