Figure 3.

GC-MS Analysis of N. benthamiana Leaf Extracts Transiently Expressing Casbene Synthase and Cytochrome P450 Enzymes.

(A) Gas chromatograph obtained with expression of casbene synthase only (lower, black), casbene synthase and CYP726A14 (middle, red), and casbene synthase plus CYP726A14 and CYP726A16 (upper, blue). The two insets display rescaled chromatographs containing the retention times at which the casbene synthase oxidation products eluted from the column.

(B) to (E) Electron ionization mass spectra for casbene (B), casbene oxidation products produced by CYP726A14 ([C] and [D]), and product obtained from coexpression of proteins encoded CYP726A14 and CYP726A16 (E). The molecular ions are indicated in red and are shown in insets for (C) and (E).