Skip to main content
. 2014 Aug 29;26(8):3286–3298. doi: 10.1105/tpc.114.129668

Figure 5.

Figure 5.

Clustering of Casbene Synthases and CYP726A Genes in J. curcas and E. peplus.

(A) Physical linkage of three casbene synthase genes (GenBank accession KJ026361), two cytochrome P450 genes (GenBank accession KF986815 and J. curcas genome database accession Jcr4S16860.10) and casbene synthase and CYP726A pseudogenes (AP011970) on chromosome 1 of J. curcas determined by linkage mapping in a population of 220 F2 individuals (47).

(B) Physical linkage of a casbene synthase homolog and CYP721A19 in E. peplus determined by long-distance PCR (GenBank accession KJ026362). The numbers under the vertical bars represent the position within the gene cluster in kilobases.

(C) Casbene 5-oxidase activity of CYP726A18 (R. communis) and CYP726A19 (E. peplus). The CYP726A genes were co-expressed with a casbene synthase gene from J. curcas (CAS1).