MKK3 Activates MPK6 in BL.
(A) Activation of MPK6 by MKK3 in BL. Immunoprecipitation assays of dark-grown Col, mkk3 mutant lines exposed to BL (30 μmol m−2 s−1) for 0, 1, or 10 min. Experimental details are the same as in Figure 2B. Values below the panel indicate quantification of each band with respect to the MPK6, loading control.
(B) MKK3-dependent activation of MPK6 is not RL specific. Immunoprecipitation assay of dark-grown Col, mkk3 lines exposed to RL (60 μmol m−2 s−1) for 0, 1, or 10 min. Experimental details are the same as in (Figure 2C). Values below the panel indicate quantification of each band with respect to MPK6, the loading control.