Figure 2.
Protein Sequences and Schematic Representation of MKK Domains.
(A) Multiple sequence alignment of Arabidopsis stomatal development-associated MKK proteins. Four variable regions differentiating MKK4/5 from MKK7/9 were examined for their contribution to signaling specificity; Next (yellow), Nint (orange), the D-domain (blue), and the C-terminal extension (green dashed line). Putative 14-3-3 binding site of MKK4 is in red. Full-length protein sequences used in alignment are provided in Supplemental Data Set 1.
(B) Schematic representation of CAMKK variant constructs (domains not to scale) designed to identify the contribution of each region to MKK signaling. The name of each construct is listed within the region depicting the canonical kinase domain of each protein.