Table 2. Summary of in Vitro and in Vivo Behaviors of CAMKK Variants.

Kinase Variant	In Vitro Activity on: (Fold over Background)		Number of Independent T1 Plants Showing Altered Stomatal Development When Expressed in Stage:			Visualize YFP in:
	MPK3	MPK6	SPCH (Inhibit)	MUTE (Inhibit)	FAMA (Clusters)	SPCH	MUTE	FAMA
MKK4 (Lampard et al., 2009)	14.7	103.6	Arrest	Arrest	Wild type	−	−	+
MKK4ΔNext	8.4	33.6	2/42	1/31	0/25	−	−	−
			4/12 T2s had paired stomata	8/13 T2s had paired stomata
MKK4ΔNint	3.1	79.5	2/48	1/67	0/30	++	++	++
			4/10 T2s had paired stomata	6/12 T2s had paired stomata
MKK4ΔNextD	1.8	2.7	0/30	0/28	0/30	++	++	++
MKK4ΔNextDNint	1.2	2.6	0/27	0/22	0/26	++	++	++
MKK4ΔCterm	2.7	91.7	6/6	4/4	0/18	−	−	++
MKK4T25N	9.0	63.8	8/10	N/A	6/8a	++	N/A	++
MKK5 (Lampard et al., 2009)	3.0	97.9	Arrest	Arrest	Wild type	−	−	++
MKK5ΔNext	5.8	74.1	1/32	2/45	0/28	+	+	+
			3/7 T2s had paired stomata	6/15 T2s had paired stomata
MKK5ΔNint	10.0	144.5	3/62	1/55	0/28	++	++	++
			9/12 T2s had paired stomata	5/12 T2s had paired stomata
MKK5ΔNextD	0.9	3.7	0/34	0/21	0/27	++	++	++
MKK5ΔNextDNint	1.0	3.8	0/36	0/24	0/31	++	++	++
MKK5ΔCterm	4.9	93.0	5/5	8/8	0/20	−	−	++
MKK7 (Lampard et al., 2009)	5.0	3.5	Arrest	Arrest	Clusters	−	−	++
MKK7ΔD	110.2	51.2	2/69	4/55	24/69b	+	+	+
MKK9 (Lampard et al., 2009)	6.8	1.2	Arrest	Arrest	Clusters	−	−	−
MKK9ΔD	24.8	5.2	2/45	2/39	17/58b	++	++	++

Some data in YFP visualization columns were taken from those presented by Lampard et al. (2009). Fluorescence scale is −, negative; +, weak; ++, strong. N/A, not applicable; this construct was not generated.

^aThese plants showed paired stomata.

^bStomatal clusters qualitatively smaller than those induced by full-length CAMKK7/9. Wild-type MKK variant phenotypes are as described (Lampard et al., 2009).