Table 1. Relative Quantification of Aromatic Amino Acids, Phenolics, Anthocyanins, and Glycoalkaloids in Semi-Polar Extracts of ABCG1-RNAi and Control Periderm.
| Compound | Log2(Peak Area/Fresh Weight) Median ± sd |
|
|---|---|---|
| Control | ABCG1-RNAi | |
| Phe | 11.9 ± 0.5 | 9.9 ± 0.8*** |
| Tyr | 12.5 ± 0.4 | 11.7 ± 1.0*** |
| Trp | 11.8 ± 0.4 | 11.4 ± 1.1 |
| Caffeic acid | 9.6 ± 0.9 | 6.7 ± 1.6*** |
| Chlorogenic acid | 14.2 ± 0.3 | 12.8 ± 0.6*** |
| Cinnamoyl putrescine | n.d. | 9.5 ± 1.3 |
| Coumaroyl putrescine | n.d. | 10.9 ± 1.5 |
| Caffeoyl putrescine | 7.4 ± 0.5 | 14.4 ± 1.4*** |
| Dihydrocaffeoyl putrescine | 7.0 ± 0.7 | 14.3 ± 1.9*** |
| Feruloyl putrescine | 9.8 ± 0.6 | 14.9 ± 1.0*** |
| Dihydroferuloyl putrescine | 9.7 ± 0.6 | 12.2 ± 1.0*** |
| Vanilloyl putrescine | 7.7 ± 0.3 | 9.3 ± 0.5*** |
| Feruloyl tyramine (FT) | 9.1 ± 0.5 | 8.3 ± 0.9*** |
| Feruloyl octopamine (FO) | 9.0 ± 1.1 | 9.5 ± 1.3 |
| FT-FT dehydrodimer | 5.4 ± 0.8 | 12.7 ± 0.8*** |
| FT-FO dehydrodimer | n.d. | 10.4 ± 1.0 |
| Pelargonidin-3-(coumaroyl-Rha-Glc)-5-Glc | 11.3 ± 0.9 | 9.5 ± 2.0*** |
| Pelargonidin-3-(feruloyl-Rha-Glc)-5-Glc | 9.7 ± 0.7 | 8.4 ± 2.0*** |
| α-Chaconine | 13.6 ± 0.3 | 13.2 ± 1.2** |
| α-Solanine | 10.6 ± 0.4 | 10.2 ± 1.0** |
Methanolic extracts of ABCG1-RNAi (line N [n = 8], line P [n = 8], line B [n = 8], line F [n = 8]) and control (wild type [n = 8] and empty vector [n = 8]) tuber periderm were prepared and analyzed by UPLC/PDA/ESI(+)-QTOFMS. Target compounds were relatively quantified using quantifier ions/retention times from Supplemental Table 11. Resulting peak areas were normalized to fresh weight and log2 transformed. Values shown represent medians ± sd. Significance analysis of differences between ABCG1-RNAi and control was performed by t test (two-tailed, unequal variances): *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001. n.d., not detectable.