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. Author manuscript; available in PMC: 2015 Mar 24.
Published in final edited form as: Biochim Biophys Acta. 2009 Jul 29;1791(12):1144–1154. doi: 10.1016/j.bbalip.2009.07.005

Fig. 3.

Fig. 3

Effect of different extracellular matrix proteins on the secretion of apoB and apoA1 by Caco-2 cells. Panels A and B: Effect of various treatments of HACT on apoB secretion. Prior to plating the Caco-2 cells, the HACT was boiled, soaked in isopropanol (isopro.), treated with trypsin, fixed in glutaraldehyde (Glut.) or freeze/thawed twice (2×F & T) to determine the effect on the secretion of apoB (A, *p<0.0001, **p<0.04) or apoA1 (B). Caco-2 cells were cultured for 14 days on the treated and untreated substrates. Basal media were collected and immediately assayed by ELISA. Average±s.d. values are depicted (n=4). Panels C and D: Effect of different extracellular matrix proteins on apoB secretion. Caco-2 cells were cultured for 21 days on uncoated Transwell® filters, filters which had been coated with laminin-1, collagen type IV or non-fibrillar collagen type I (non-fibrous Col 1), and on HACT (Amnion CT). The basal medium was exchanged with fresh DMEM containing 1% FBS. After 18 h, basal medium was collected and immediately assayed by ELISA for apoB (C, *p<0.0001) or apoA1 (D). Average±s.d. values are plotted (n=4). Panel E: Effect of fibrillar and non-fibrillar collagen type I on the secretion of apoB by Caco-2 cells. Caco-2 cells were cultured for 14 days on uncoated Transwell® filters, filters coated with non-fibrillar or fibrillar collagen type I, or on HACT (Amnion CT). Basal medium was collected and immediately assayed by ELISA for apoB (*p<0.0005, **p<0.008). Panel F: Effect of different amounts of fibrillar collagen type I on the secretion of apoB by Caco-2 cells. Caco-2 cells were cultured for 14 days on uncoated Transwell® filters, or filters with different amounts of fibrillar collagen type I added to the cylinder. Basal medium was collected and immediately assayed by ELISA for apoB as described before (*p<0.001, **p<0.02, ***p<0.03). Average±s.d. values are depicted (n=4).