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. Author manuscript; available in PMC: 2015 Mar 24.
Published in final edited form as: Biochim Biophys Acta. 2009 Jul 29;1791(12):1144–1154. doi: 10.1016/j.bbalip.2009.07.005

Fig. 6.

Fig. 6

Effect of extracellular matrix proteins on the TER and apoB secretion by Caco-2 cells. Panels A and B: Caco-2 cells were cultured for 14 days on Transwell® filters coated with laminin-1, collagen type IV, non-fibrillar collagen type I, or HACT (Amnion CT). Uncoated filters (Transwell) served as controls. The basal medium was exchanged with fresh DMEM containing 1% FBS and, 18 h later, was collected and immediately assayed by ELISA for apoB (*p<0.005, Transwell vs Amnion CT, n=4, A). The TER was assessed on day 14 in DMEM+10% FBS (**p<0.005 Transwell vs Laminin-1, ***p<0.01 Transwell vs Collagen IV, n=4, B). Panels C and D: Caco-2 cells cultured for 14 days on uncoated Transwell® filters or on amnion connective tissue were incubated for 18 h with fresh DMEM containing 1% FBS±50 μM PD98059 (****p<0.0001 Transwell® vs Transwell®+PD98059, n=6, C) or mAb AIIB2 (D) and used to assess TER in DMEM+10% FBS. Panels E and F: Correlation between apoB secretion and TER. Caco-2 cells were cultured for 14 days on Transwell® filters or on HACT. The basal medium was changed to fresh DMEM containing 1% FBS, collected after 18 h, and immediately assayed for apoB. The cultures were then assessed for TER in DMEM+10% FBS. Paired values of apoB and TER from 45 cultures from 12 independent experiments are depicted for Transwell® filters (E) and HACT (F). Linear regression slope is not significantly different from zero (Transwell® filters p=0.7792, Amnion CT p=0.2482).