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. 2015 Mar 17;16(1):200. doi: 10.1186/s12864-015-1411-x

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© Li et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression patterns of SmWRKY genes in roots (Rt), stems (St), leaves (Le) and flowers (Fl) of 2-year-old, field-grown S. miltiorrhiza Bunge (line 993). The expression level of SmWRKYs was analyzed by the quantitative RT-PCR method. Y-axis indicates relative expression levels. X-axis indicates different tissues. SmUBQ10 was used as the reference gene. Transcript levels in leaves were arbitrarily set to 1 and the levels in other tissues were given relative to this. Error bars represent standard deviations of mean value from three biological and three technical replicates. ANOVA (analysis of variance) was calculated using SPSS. P < 0.05 was considered statistically significant.