Fig. 2.

Gonadal sex in the different treatments. Overall sex ratios for each treatment combination (A) and proportion of females by individual treatment (B) for n = 18 inbred and n = 20 outbred zebrafish families (each stocked with n = 4 individuals per treatment). Boxes represent the interquartile range and median (line), and whiskers show the full range, excluding outliers. The fixed effects of breeding, clotrimazole exposure, and temperature all contributed significantly to reductions in the proportion of females (arcsine square root-transformed) per family per treatment (all P ≤ 0.0003) according to the best-fit lme model (SI Appendix, Table S1.2).