Fig. 3.

Germ cell progression in individuals: females (A) and males (B) for n = 18 inbred and n = 20 outbred zebrafish families (each stocked with n = 4 individuals per treatment). Individuals were classified by their most advanced germ cell developmental stage (advancing stages 1–3 are indicated by increasingly darker shading). According to the best-fit lme model (SI Appendix, Table S1.4), there were significant differences, due to breeding and gonadal sex (both P ≤ 0.0017), in germ cell development in inbred males (79 ± 0.8% spermatids) vs. outbred males (91 ± 0.4% spermatids), but not in inbred females (42 ± 3.4% ≥cortical alveolar stage) compared with outbred females (44 ± 2.8% ≥cortical alveolar stage).