Table 1.

Experimental treatments

Clotrimazole nominal (and measured) exposure concentrations, μg/L	Inbred zebrafish families (FIT = n + 0.25)		Outbred zebrafish families (FIT = n)
	28 °C	33 °C	28 °C	33 °C
0 (0)	✔	✔	✔	✔
2 (1.7)	✗	✔	✗	✔
10 (8.0)	✔	✔	✔	✔

F_IT = n (unknown quantity) for outbreds and F_IT = n + 0.25 for inbreds following one generation of full-sibling mating.

✔Replication for each treatment was n = 18 inbred families and n = 20 outbred families. Each family replicate per treatment was represented by n = 4 fish.

✗This “low dose × low temperature” treatment was not included in our analyses because we knew, from our previous work (40, 83), that the low-dose clotrimazole (2 μg/L) alone would not affect population-relevant end points (survival, growth, gonadal development or fecundity), and we sought to maximize replication for the other treatment regimes.