Table 2.

Model scenarios based on sex ratio skews in combined clotrimazole exposure, temperature, and breeding treatments

No.	Emergent inbreeding depression	Exposure scenario	Age 0+ survivorship	Overall proportion of females per treatment, %	Mean proportion of females per family per tank compartment per treatment, % (SD)
1a	✔	Inbred, zero clotrimazole, 28 °C	Low	42	41 (28)
1b	✗	(Inbred control)
2a	✔	Outbred, zero clotrimazole, 28 °C	High	57	58 (30)
2b	✗	(Outbred control)
3a	✔	Inbred, zero clotrimazole, 33 °C	Low	27	28 (33)
3b	✗
4a	✔	Outbred, zero clotrimazole, 33 °C	High	52	52 (34)
4b	✗
5a	✔	Inbred, high clotrimazole, 28 °C	Low	19	18 (18)
5b	✗
6a	✔	Outbred, high clotrimazole, 28 °C	High	40	40 (30)
6b	✗
7a	✔	Inbred, low clotrimazole, 33 °C	Low	18	18 (22)
7b	✗
8a	✔	Outbred, low clotrimazole, 33 °C	High	51	51 (26)
8b	✗
9a	✔	Inbred, high clotrimazole, 33 °C	Low	3	3 (8)
9b	✗
10a	✔	Outbred, high clotrimazole, 33 °C	High	18	17 (26)
10b	✗

Exposure scenarios represent treatment combinations in the exposure study. Clotrimazole geometric mean measured concentrations: control (0 μg/L), low (1.7 μg/L), high (8 μg/L). First-year (age 0+) survivorship: low = 4 ± 3% for inbreds, high = 9 ± 3% for outbred zebrafish (SI Appendix, Table S7). Proportions of females per family (tank compartment) per treatment were used for statistical analysis (arcsine-square root transformed data) and PVA.