Table 2.
No. | Emergent inbreeding depression | Exposure scenario | Age 0+ survivorship | Overall proportion of females per treatment, % | Mean proportion of females per family per tank compartment per treatment, % (SD) |
1a | ✔ | Inbred, zero clotrimazole, 28 °C | Low | 42 | 41 (28) |
1b | ✗ | (Inbred control) | |||
2a | ✔ | Outbred, zero clotrimazole, 28 °C | High | 57 | 58 (30) |
2b | ✗ | (Outbred control) | |||
3a | ✔ | Inbred, zero clotrimazole, 33 °C | Low | 27 | 28 (33) |
3b | ✗ | ||||
4a | ✔ | Outbred, zero clotrimazole, 33 °C | High | 52 | 52 (34) |
4b | ✗ | ||||
5a | ✔ | Inbred, high clotrimazole, 28 °C | Low | 19 | 18 (18) |
5b | ✗ | ||||
6a | ✔ | Outbred, high clotrimazole, 28 °C | High | 40 | 40 (30) |
6b | ✗ | ||||
7a | ✔ | Inbred, low clotrimazole, 33 °C | Low | 18 | 18 (22) |
7b | ✗ | ||||
8a | ✔ | Outbred, low clotrimazole, 33 °C | High | 51 | 51 (26) |
8b | ✗ | ||||
9a | ✔ | Inbred, high clotrimazole, 33 °C | Low | 3 | 3 (8) |
9b | ✗ | ||||
10a | ✔ | Outbred, high clotrimazole, 33 °C | High | 18 | 17 (26) |
10b | ✗ |
Exposure scenarios represent treatment combinations in the exposure study. Clotrimazole geometric mean measured concentrations: control (0 μg/L), low (1.7 μg/L), high (8 μg/L). First-year (age 0+) survivorship: low = 4 ± 3% for inbreds, high = 9 ± 3% for outbred zebrafish (SI Appendix, Table S7). Proportions of females per family (tank compartment) per treatment were used for statistical analysis (arcsine-square root transformed data) and PVA.