Identification of 20 opsin genes in three dragonflies of the family Libellulidae. (A) A neighbor-joining phylogeny of 20 opsin genes each from S. frequens (red shade), O. albistylum (blue shade), and L. fulva (gray shade) inferred from 869 aligned amino acid sites. On each node, bootstrap values are indicated in the order of neighbor-joining method/maximum-likelihood method. Accession numbers or annotation IDs are shown in brackets. Classification of the opsin genes is indicated on the right side. Insect visual opsin genes are highlighted in purple for UV type, in blue for SW type, and in green for LW type, respectively. (B) Gene clusters of SW and LW opsin genes on the genome of L. fulva.