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. 2015 Mar 4;112(11):E1333–E1342. doi: 10.1073/pnas.1424341112

Fig. 5.

Fig. 5.

NYVAC-C Δ3 enhances the magnitude and polyfunctionality of the CD8 T-cell response to HIV-1 Gag–Pol antigens. Vaccine-induced HIV-1–specific CD8 T-cell response in mice (n = 4 per group) infected with 107 PFUs of NYVAC-WT, NYVAC-C, or NYVAC-C Δ3. The response was measured 11 d after the last immunization, after splenocyte stimulation with HIV-1 peptides/pools or with A20 GPN+. Total value (magnitude) is the sum of total CD8 T cells per spleen that secrete IFN-γ and/or TNF-α and/or IL-2 and/or CD107a. (A) Magnitude of Pol-1–, Pol-2–, or Gag pool-specific CD8 T-cell response. Graphs show mean ± CI. (B) Magnitude of CD8 T-cell response to A20 GPN+. Graphs show mean ± CI. (C) Functional profile of adaptive Pol-1 + Pol-2–specific CD8 T cells. (D) Functional profile of adaptive Gag pool-specific CD8 T cells. (E) Functional profile of adaptive GPN-specific CD8 T cells. Combinations of responses (x axis) and total numbers of functionally distinct cell populations (y axis) are shown. Responses are grouped and color-coded based on the number of functions. Pie chart colors indicate the percentage of cytokine-producing cells based on number of functions (inside) and the different activation markers (outside). (F) Absolute number of Nα and Nβ neutrophils in the peritoneal cavity at 6 h postinfection in NYVAC-C– or NYVAC-C Δ3- (107 PFUs) injected and IgG2A-pretreated or 1A8-pretreated mice. Boxes with dashed lines indicate absolute number in PBS-injected mice. Graphs show mean ± SEM; each point represents an individual mouse. (G) Vaccine-induced HIV-1–specific CD8 T-cell response in mice (n = 4 per group) infected with 107 PFUs NYVAC-WT, NYVAC-C, or NYVAC-C Δ3 and IgG2A-pretreated or 1A8-pretreated. The response was measured 11 d after the last immunization, after splenocyte stimulation with HIV-1 peptides/pools. Total value (magnitude) is the sum of total CD8 T cells per spleen that secrete IFN-γ and/or TNF-α and/or IL-2 and/or CD107a. Graphs show mean ± CI. Data are representative of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.