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. 2015 Feb 2;112(11):E1392–E1400. doi: 10.1073/pnas.1424840112

Fig. 3.

Fig. 3.

Enzyme activities and protein-based complementation in WT, trxo1, and ntra ntrb mutants. CS, SCoAL, MDH, FUM, and SDH activities were measured in mitochondrial extracts, whereas ACL was measured in leaf extracts of WT, trxo1, and ntra ntrb mutants. These extracts were also used to perform protein-based complementation assays. The extracts were untreated (Control) or treated with TRXo1 (3 μg; 180 nM) or TRXh2 (3 μg; 210 nM) both reduced by NTRB (7.5 μg; 100 nM) and NADPH (100 μM). Data presented are mean ± SEM (n = 5). A number symbol indicates values significantly different from WT by the Student’s t test at 5% (#P < 0.05), and an asterisk indicates values significantly different from the control in the same genotype by the Student’s t test at 5% (*P < 0.05).