Fig. 3.
Identification of the interaction preferences between Gαi1βγ heterotrimer combinations and NTR1 or TM86V L167R. (A) Heterotrimer combinations consisting of αi1, β1–4 and HA-γ1–13 were tested for their interaction with NTR1 WT receptor in the presence of 20 µM NT in a coimmunoprecipitation experiment directed against the HA tag of γ. The results are presented in form of Western blots against NTR1 and the αi1-subunit. As a visual guide to assess the potency of GPCR/G-protein complex formation, the ratio of signal intensities between GPCR and αi1-subunit are given below the blots. Bar heights indicate the interaction preference of a given G-protein combination for the GPCR in question, with higher being better. (B) Interaction studies for TM86V L167R as in A.