Figure 5.

In vivo imaging.

Notes: The MCF-7/ADR tumor-bearing mice received an intravenous injection, via the tail vein, of physiological saline (A), cy7-LTSL (B), and cy7-LTSL-CREKA (C) at a dose of 750 μg/kg. Other tumor-bearing mice were intravenously injected heparin solution (a bolus of 800 units/kg), followed 120 minutes later by cy7-LTSL-CREKA (750 μg/kg), and received additional heparin by intraperitoneal injections (a total of 1,000 units/kg) throughout the experiment (D). All mice were then anesthetized with intraperitoneal injection of pentobarbital (60 mg/kg), and scanned at 2 hours, 6 hours, 8 hours, and 12 hours using a Kodak In Vivo Imaging System FX PRO.(p)(p)Abbreviations: cy7, carbocyanine dye; LTSL, lysolipid-containing thermosensitive liposome; CREKA, Cys-Arg-Glu-Lys-Ala.