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. 2015 Jan 22;3(1-2):e983777. doi: 10.4161/21688370.2014.983777

Figure 1.

Figure 1.

Regulatory role of Interleukin-9 in TNBS-mediated colitis model. Wild-type and IL-9-deficient animals were subjected to the TNBS colitis model (A). The manifestation of colitis was monitored using mini-endoscopy and endoscopic scoring of colitis activity. Endoscopic score was calculated regarding 5 parameters of inflammation (fibrin, translucent, granularity, vascularity and stool). Analysis of the body weight was done at indicated time points and significant differences are marked. IVIS analysis with Luminol detected the free radical production during inflammation in WT and IL-9 KO mice. Finally, histopathologic sections revealed the assessment of colitis in both groups. Data represent results of 3 independent experiments. Cryosections from TNBS-treated WT and IL-9 KO mice were stained with a specific anti PU.1 antibody. Cell nuclei were counterstained with HOECHST dye (B). Representative stainings and statistical analysis are shown. In addition, double staining with anti CD4 (green) and anti PU.1 (red) was performed and significant changes are indicated. For this analysis 4 different animals per group were used.