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. 2015 Feb 3;11(2):e1004901. doi: 10.1371/journal.pgen.1004901

Figure 3. Independent bouts of inflammation induce DSB formation but not HR.

Figure 3

(A) Immunohistochemical staining for the DSB marker γH2AX (yellow) in pancreas sections. Nuclei were counterstained with DAPI (blue). In control mice, nuclei with γH2AX foci are very rare (Left). However, nuclei with γH2AX foci (arrowhead) appear after independent bouts of inflammation (Right). (B) Quantification of nuclei containing more than five γH2AX foci shows significantly more γH2AX positive nuclei after inflammation (n = 6) than in control animals (n = 6). Data are mean ± SEM. *** P < 0.001 (Student’s t-test). (C) Numbers of fluorescent foci in the pancreas are not different between control mice (n = 17) and mice that underwent repeated acute inflammation (n = 17). Symbols represent data from individual mice, horizontal bars show medians. ns, not statistically significant (Mann–Whitney U-test). (D) No statistically significant difference in the frequencies of fluorescent cells in the pancreas between control mice (n = 17) and mice that underwent repeated acute inflammation (n = 17). Pancreata were disaggregated into single-cell suspensions and the frequencies of fluorescent cells were determined by flow cytometry. Symbols represent data from individual mice, horizontal bars show median values. ns, not statistically significant (Mann–Whitney U-test).