Figure 7. Simultaneous inflammation and cell proliferation induces HR in the pancreas.

(A) Representative images from pancreata of control mice (Top) and mice that experienced combined proliferation and inflammation (Bottom). Freshly harvested whole organs were compressed between glass coverslips and imaged under an epifluorescent microscope. Representative details of composite images are shown, fluorescent foci are apparent in situ. More foci are visible in the pancreas from the proliferation plus inflammation group. Brightness and contrast have been enhanced identically. (B) Numbers of fluorescent foci are higher in mice that experienced combined proliferation and inflammation (n = 18) than in control mice (n = 17). Symbols represent data from individual mice, horizontal bars show medians. **, P < 0.01, (Mann–Whitney U-test). (C) Higher fluorescent cell frequency in the pancreata of mice that experienced combined proliferation and inflammation (n = 18) than in control mice (n = 17). Pancreata were disaggregated into single-cell suspensions and the frequencies of fluorescent cells were determined by flow cytometry. Symbols represent data from individual mice, horizontal bars show median values. *, P < 0.05 (Mann–Whitney U-test).