Fig. 5.

DPDPE, but not TAN-67, induced translocation of β-arrestin 2-GFP. HEKδ cells were transiently transfected with 0.4 μg β-arrestin 2-GFP and grown on poly-D-Lys coated 12 mm cover slips. The transfected cells were treated overnight with, or without, 100 ng/ml PTX, then incubated with 10 μM DPDPE or TAN-67 for 5 min at 37 °C. Visualization of FLAG δ-opioid receptor and β-arrestin 2-GFP by confocal microscopy was as described in Materials and methods.