Figure 3.

Chemical cross-linking of the FMO/RCC complex by different cross-linkers (10 mM) and identification of subunit interactions by LC–MS/MS: (A) DTSSP-cross-linked sample treated with (lane 1) and without (lane 2) a reducing agent, markers (lane 3), and non-cross-linked control sample (lane 4). (B) BS³-cross-linked sample treated with (lane 3) and without (lane 4) a reducing agent and a sample cross-linked on ice and treated without a reducing agent (lane 5) and DSS-cross-linked sample treated with (lane 7) and without (lane 8) a reducing agent, markers (lanes 1 and 6), and non-cross-linked control sample (lane 2). (C) MS/MS spectrum of the interlinked peptide between PscA and PscC induced by BS³.