Figure 2.

Effects of the chimeric compounds (R)-7–(R)-10 on inactivation state of Na⁺ currents in CAD cells. (A) Inactivation voltage protocol. Currents were elicited by 5 s prepulses between −120 and −20 mV and then fast-inactivated channels were allowed to recover for 150 ms at a hyperpolarized pulse to −120 mV. The fraction of channels available at 0 mV was analyzed. (B) Representative current traces from CAD cells without (control, 0.1% DMSO) or in the presence of 10 μM of the compounds as indicated. The red and blue traces represent the currents evoked at −120 and −50 mV (arrows), respectively (also highlighted in the voltage protocol as a dashed line). (C–F) Summary of inactivation curves for CAD cells treated with 0.1% DMSO (control) or various concentrations of the compounds as indicated. The concentrations of half maximal effect for −50 mV conditioning pulse (see text for detailed explanations), the IC₅₀, are indicated in boxes within each panel. Data are from 4 to 8 cells per condition. Some error bars are smaller than the symbols.