Figure 7.

Effects of the chimeric compounds ((R)-1, (R)-4–(R)-10) and the parent compounds ((R)-2, (S)-3) on fast inactivation and steady-state activation states of Na⁺ currents in rat embryonic cortical neurons. Voltage protocol for fast inactivation (top left) and steady-state activation (top right). Representative Boltzmann fits for steady-state fast inactivation and steady-state activation for cortical neurons treated with 0.1% DMSO (control) and various concentrations of the indicated compounds are shown. Values for V_1/2, the voltage of half-maximal inactivation and activation, and the slope factors (k) were derived from Boltzmann distribution fits to the individual recordings and averaged to determine the mean (±SEM) voltage dependence of steady-state inactivation and activation, respectively. Statistically significant differences between control and fast inactivation or steady-state activation are indicated by the asterisks in symbol key (*p < 0.05, one-way ANOVA; n = 5–7 cells per condition).