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. 2014 Jul 8;16(14):3652–3655. doi: 10.1021/ol501609y

Figure 3.

Figure 3

Macrocyclization of peptides with different perfluoroaromatic linkers. Reaction conditions: 0.1 mM peptide 1a–c, 0.2 mg/mL GST, 20 mM tris(carboxylethyl)phosphine hydrochloride (TCEP·HCl), 0.1 M phosphate, pH 8.0, room temperature (25 °C), 5 min. (A) Reaction yields with different linkers with or without the addition of GST. (B) LC–MS analysis of crude reaction mixtures with peptide 1a. Amino acids are shown in one-letter codes. γ-E: γ-glutamic acid. LC–MS data shown are total ion currents (TIC); mass spectra at the highest points of the TIC peaks are shown as insets.