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. 2014 Jul 8;16(14):3652–3655. doi: 10.1021/ol501609y

Figure 4.

Figure 4

Macrocyclization of peptides with increasing lengths. Reaction conditions: 0.1 mM peptide 2a–4a, 0.2 mg/mL GST, 20 mM TCEP·HCl, 0.1 M phosphate, pH 8.0, room temperature (25 °C), 2 h. (A) Reaction yields of cyclization reactions with and without the addition of GST (C(S-tBu): tert-butylthio-protected cysteine; C*: perfluoroaryl-modified cysteine). The two cysteines that were cross-linked together are highlighted in red. (B) LC–MS analysis of reactions of peptide 2a to 4a with the addition of GST. LC–MS data shown are total ion currents (TIC); mass spectra at the highest points of the TIC peaks are shown as insets.