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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cell Mol Bioeng. 2014 Dec 9;8(1):160–177. doi: 10.1007/s12195-014-0370-7

Figure 4.

Figure 4

Effect of ROCK inhibition on stretch-induced edge ruffling. (A) EGFP-actin dynamics in an EC pretreated with Y27632 and subjected to cyclic uniaxial stretch in the presence of the inhibitor. Rose plots show the angular distribution of edge ruffles in during a 15-min measurement window. Arrows indicate stretch directions. Scale bars, 20 μm. (B) Mean ruffling perimeter fraction, computed as the ratio of the number of angular bins engaged in ruffling to the total number of angular bins. (C) Circular variance computed from the angular distribution of mean ruffling orientations of individual cells. Rose plots show mean ruffling orientations of individual ECs at indicated times. Blue bar indicates mean ruffling orientation across multiple cells. (*, p < 0.05, v-test, unimodal alternative of 0°) (n = 17 cells from separate experiments)