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. 2015 Mar 19;7(1):31. doi: 10.1186/s13148-015-0058-4

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© Vieweg et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Pyrograms showing methylation level for each cytosine within the investigated sequences in spermatozoa of one fertile donor and two selected infertile patients. In contrast to the hypomethylated promoter region of NSD1, paternally imprinted and hypermethylated H19 locus is presented as a control. Pyrosequencing using fully methylated and fully unmethylated DNA was carried out for the internal control.