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. 2015 Mar 19;7(1):31. doi: 10.1186/s13148-015-0058-4

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© Vieweg et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunofluorescent labeling of H4K12ac and 5-methylcytosine (5mC) in mouse sperm nucleus. Double stained spermatozoa with anti-H4K12ac antibody (green) (A), anti-5mC antibody (red) (B), merged with DAPI (C), and merged with DIC (D).