Table 4.

Primers designed for pyrosequencing

Gene	Primers		Sequence to analyze	PCR product size (bp)
PRM_1	F	5′-GGG GTG GTT AGG GAT ATG T-3′	ATTTYGTGYGAGYGTTYGTTTAGGTTTTTTAYGYGGTATYGGATTATGGTGTTGGGAGATTTGGTGG	335
	R	Bio5′-CAC CAA ATC TCC CAA CAC CAT-3′
	S	5′-ATG TAA TTG TTG TTT GTA T-3′
PRM_2	F	Bio5′-GGG GTG GTT AGG GAT ATG T-3′	ACCRAATCCACAAACRACAACATCRCTCCTACAAAAAACRCAAAAAACRCTCCTACAAACACC	182
	R	5′-AAA AAC CCA TAA CCA ATC TCA CTA TAA-3′
	S	5′-CAC TAC TCT CCA AAA AAA CTA C-3′
AFF4	F	5′-GGG TTA GGG TTG GGA TAG TTG A-3′	TTYGTTTYGTTYGTTGGYGGYGGYGAYGGTAGTTGGATTTTTGTAGTTAGGGT	229
	R	Bio5′-AAA CCC CCC CCC CCT ACT-3′
	S	5′-GAA GAT TTG GTA TTA GGA TT-3′
H19_4	F	5′-GGGGGTTTTTGTATAGTATATGGGT-3′	AATYGGAAGTGGTYGYGYGGYGGTAGTGTAGGTTTATATATTATAGTT	242
	R	Bio5′-ACTCCTATAAATATCCTATTCCCAAAT-3′
	S	5′-GGAATTGGTTGTAGTTGTGG-3′
NSD1m1	F	5′-AGTTGAGGATTTAGTAGGTTTGTATTTAG-3′	GTGTAGTYGTTTYGGTYGGTTYGTTTGYGGTTTGYGTATYGTYGTTGTAAAGGTTT	173
	R	Bio5′-TACCCCCCCCTTCCTAACT-3′
	S	5′-GGAAGGAGAGTTTTGGG-3′

Table shows sequence to analyze and size of the PCR amplicons. F: forward primer; R: reverse primer; S: sequencing primer; Bio: biotinylation.