Fig 7. β-catenin in the renal stroma modulates Wnt9b expression in ureteric epithelial cells.

(A-C) When compared to WT, In situ hybridization and real-time quantitative PCR for Wnt9b demonstrates Wnt9b mRNA expression is significantly reduced (1.00 versus 0.29, p=0.008) in E14.5 β-cat^S-/- kidneys. (D-I) In situ hybridization and Real-time quantitative PCR for Ret and Wnt11 demonstrated no differences in mRNA expression between WT and β-cat^S-/- at E14.5. (J-K) Histological analysis of β-cat^GOF-S mutant kidneys demonstrate a marked increase in condensing mesenchyme population when compared to WT at E14.5. (L-N) In situ hybridization and quantitative PCR demonstrate Wnt9b expression in β-cat^GOF-S kidneys was significantly increased (1.02 versus 3.102, p=0.021) as compared to WT at E14.5. (scale bar = 50 μm, rc-renal capsule, cm= condensing mesenchyme, ub = ureteric epithelium).