Fig 3. Th2-type cellular and humoral immune responses are decreased in JH^-/- mice.

WT or JH^-/- mice were intranasally exposed to OAAH or PBS for 2 weeks, as described in Fig. 1A. (A) Cytokine levels in lung homogenates were determined by ELISA. (B) Blood was collected from these mice 24 hours after the last allergen exposure. The antibody levels in the plasma were analyzed by ELISA. (C) Single-cell suspensions of the lung were stained with anti-CD3 and anti-CD4 antibodies and analyzed by flow cytometry. Cell numbers were calculated based on cell counts and percentages of CD3⁺ and CD4⁺ cells. Results are presented as mean±SEM (n = 5 mice per group). *, p<0.05; **, p<0.01, between the groups indicated by horizontal lines. Data are representative of two independent experiments.