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. 2015 Mar 15;13:91. doi: 10.1186/s12967-015-0440-1

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© Kong et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TS inhibits RANKL-induced TNF-α s secreted by RAW 264.7 cells. Confluent RAW 264.7 cells were incubated with or without TS (0.1, 0.5 and 2.5 μg/mL) for 2 h, followed by stimulation with RANKL (50 ng/mL) for 24 h, and supernatants were analyzed for TNF-α by enzyme-linked immunosorbnent assay (ELISA). Data are represented as the mean ± SD of three independent experiments. ^## P < 0.001 significantly different from control. **P < 0.01 and ***P < 0.001 significantly different from RANKL only group.