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. 2015 Mar 15;13:91. doi: 10.1186/s12967-015-0440-1

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© Kong et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TS abrogates expression of TRAF6 in RANKL-induced RAW 264.7 cells. RAW 264.7 cells were pre-treated with RANKL (50 ng/mL), and cell lysates were collected at the time points indicated (A) and/or various concentrations of TS (0.1, 0.5 and 2.5 μg/mL) for 24 h. The harvested cells from triplicate tests were subjected to western blot analysis for TRAF6 (B). Relative amount of protein were determined by densitometric analysis. One of three experiments with similar results is shown. Data are represented as the mean ± SD. ^### P < 0.001 significantly different from control. *P < 0.05, **P < 0.01 and ***P < 0.001 significantly different from RANKL only group.