Table 1. HPLC elution scheme and solvents used in the separation of phytoplankton pigments.

	Time, min
	0	2	30	43	50
Solvent A, %	50	50	100	100	50
Solvent B, %	50	50	0	0	50

Solvent A = 80% methanol: 20% 0.5 M ammonium acetate (pH 7.2) (v:v). Solvent B = 80% methanol: 20% acetone (v:v).