Fig 1. Femur biomechanical properties, according to the three-point bending test, of F2 female rats after three generations of continuous drinking of four water types.

The basic bone parameters, including length, cortical bone area, bone diameter and bone cross-sectional moment of inertia, are shown in A, B, C, and D, respectively; the bone structural properties, including ultimate load, elastic load, maximum deflection of femoral diaphysis and elastic deflection, are shown in E, F, G, H, respectively; the bone material properties, including Young modulus, ultimate stress, ultimate strain and fracture strain, are shown in I, J, K, L, respectively. TW: Tap water, bNW: bottled natural water, bMW: bottled mineralized water, bPW: bottled purified water.